052065
BMP-2/BMP-4 (Bone Morphogenetic Protein 2/Bone Morphogenetic Protein 4)
Size
£882.00
- SKU:
- 052065
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant. Endotoxin: ≤0.1EU/ug (LAL).
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Gln283-Arg396 from human BMP-2, expressed in CHO cells
- Clone:
- 24H2
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Bone Morphogenetic Protein 2 (BMP-2) and BMP-4 are two of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF-beta ) superfamily, BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. Applications: Suitable for use in Neutralization and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml with recombinant human BMP2 or recombinant human BMP4, under non-reducing conditions only Neutralization: Measured by its ability to neutralize BMP2 induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The Neutralization Dose (ND50) is typically 5-15ug/ml in the presence of 1ug/ml recombinant human BMP2 and 2ug/mL heparin. Also neutralizes human BMP4 induced activity in the ATDC5 mouse chondrogenic cell line. The ND50 is ~3ug/ml in the presence of 75ng/ml of recombinant human BMP4. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice