052040
BAFF (BLyS, CD257, TALL1, THANK, TNFSF13B, ZTNF4)
Size
£876.00
- SKU:
- 052040
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Ala127-Leu309 from mouse BAFF, expressed in NS0 cells.
- Clone:
- 121808
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- BAFF (also known as TALL1, BLyS, and THANK) is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated TNF superfamily member 13B (TNFSF13B). Mouse BAFF is a 309 amino acid (aa) protein consisting of a 248aa extracellular domain, a 21aa transmembrane region and a 45 aacytoplasmic tail. BAFF has the typical structural characteristics of TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain. BAFF is expressed in peripheral blood mononuclear cells, in spleen and lymph nodes. Its expression in resting monocytes is up-regulated by IFN-alpha, IFN-beta, LPS, and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug labels 1x10e6 cells using mouse splenocytes Western Blot: 1ug/ml using recombinant mouse BAFF/BLyS/TNFSF13B Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice