052026-HRP
Histone H3, Citrulline R2, R8, R17 (HRP)
Size
£837.00
- SKU:
- 052026-HRP
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Histone H3 peptide with citrulline at R2, R8, and R17
- Clone:
- 11D3
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.05% ProClin300, proprietary stabilizers, 50% glycerol. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- Histone H3 is a nuclear protein and a component of the nucleosome core that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications, including citrullination. Histone H3 is subject to citrullination of arginine residues by peptidyl arginine deiminase 4 (PAD4) at positions 2, 8, 17, and 26, and this citrullination blocks methylation of these residues by protein-arginine methyltransferase 4 (PRMT4) and induces transcriptional repression of target genes. Citrullinated histone H3 is a component of neutrophil extracellular traps (NETs) that acts as an autoantigen to induce the production of anti-citrullinated protein antibodies associated with various diseases such as sepsis, multiple sclerosis, rheumatoid arthritis, and multiple myeloma. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. ELISA: 1:1000. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice