052021
CD146 (MUC18, Muc-18, MCAM, Mel-CAM, S-endo, Melanoma Cell Adhesion Molecule) (APC)
Size
£576.00
- SKU:
- 052021
- Application:
- Flow Cytometry
- Purity:
- Purified by size-exclusion chromatography.
- Storage Conditions:
- 4°C Do not freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Cultured human umbilical cells.
- Clone:
- P1H12
- Format:
- Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with Allophycocyanin (APC).
- Extra Details:
- CD146, also known as MCAM (melanoma cell adhesion molecule) or MUC18, is a heavily glycosylated transmembrane glycoprotein with more than 50% of the mass from carbohydrates. It is expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, activated T cells and activated keratinocytes, and on some cancer cells, especially melanoma. The presence of CD146 on circulating blood cells has been confined to the activated T cells rather than circulating endothelial cells. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells in 100ul or 100ul whole blood Optimal dilution determined by the researcher. Excitation Laser: Red (633nm) Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice