051994
Claudin 4 (CLDN4) (PE)
Size
£735.00
- SKU:
- 051994
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met1-Val209 from human Claudin 4 expressed in NS0 cells. Species Sequence Homology: mouse and rat, 83%
- Clone:
- 7H177
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- Claudin 4 is a multipass membrane protein in the claudin family of tight junction proteins. Claudin 4 is a 209 amino acid molecule with four transmembrane segments. It is preferentially expressed in intestinal mucosa epithelium and is involved in epithelial tissue development. It is also upregulated in several carcinomas. Phosphorylation of claudin 4 regulates paracellular epithelial permeability. Human claudin 4 shares 83% aa sequence identity with mouse and rat claudin 4. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul/10e6 cells in PC-3 human prostate cancer cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice