051979

Granzyme A

Size

£460.00

SKU:: 051979
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Full length human Granzyme A.
Clone:: CB9
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: Granzyme A is a serine protease expressed in the cytoplasmic granules of T cells and NK cells. Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity. Similarly to granzyme B, granzyme A acts to destroy the target cells by proteolysis of their particular components. In case of granzyme A the targets are e.g. APEX1 (it destroys its oxidative repair activity), and nucleosome assembly protein SET (it disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA). Applications: Suitable for use in Immunocytochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5-10ug/ml using intracellular staining. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.