Heparan Sulfate Proteoglycan (Perlecan)

SKU:

051960

Purity:

Purified by Protein G affinity chromatography.

Storage Conditions:

-20°C

Supplier:

United States Biological

Host:

Mouse

Immunogen:

Full length recombinant protein corresponding to human Perlecan.

Clone:

5D7-2E4

Format:

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

Extra Details:

Perlecan is one of the extracellular matrix forms of heparan sulfate proteoglycans (HSPGs). It is a large multidomain and binds to and cross-links many extracellular matrix (ECM) components and cell-surface molecules. Perlecan is synthesized by vascular endothelial and smooth muscle cells and deposited in the extracellular matrix within all mammalian tissues. HSPGs differ in their protein cores that are thought to determine the location of HSPG in either the cell membrane (syndecan and glypican) or the extracellular matrix (perlecan and dystroglycan). HSPGs found in the brain are dystroglycan, N-syndecan glypican, and perlecan. The composition of HS isolated from brain differs significantly from that of other organs and is developmentally regulated. Thus, differences in protein cores, disaccharide composition, the extent and position of sulfation, and the number of GAG chains diversify chemical structure and functions of HSPGs. The postulated functions of HSPG include cell proliferation, differentiation, adhesion, migration, and morphogenesis. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:300 detected Perlecan in fresh-frozen normal human tonsil tissue. Optimal dilutions to be determined by the researcher. Positive Control: Fresh-frozen normal human tonsil tissue Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.