051955
Strep II-Tag (NWSHPQFEK) Epitope Tag
Size
£704.00
- SKU:
- 051955
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- NWSHPQFEK peptide conjugated to KLH
- Clone:
- 5A9F9
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 200ul sterile ddH2O.
- Extra Details:
- Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It is widely used in numerous molecular biological protocols due to its strong affinity for biotin. NWSHPQFEK (Strep tag II) is a nine amino acid peptide with high specificity and affinity towards streptavidin. NWSHPQFEK Tag Antibody is a useful tool in analysis and affinity purification of Strep tag II fusion proteins. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Flow Cytometry, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilutions: ELISA: 0.001-0.002ug/ml Western Blot: 0.1-0.5ug/ml Immunofluorescence (IC): 1-3ug/ml Flow Cytometry: 1ug/ml Immunoprecipitation: 1-5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice