051950
PTH (Parathyroid Hormone, Parathormone, Parathyrin)
Size
£825.00
- SKU:
- 051950
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ser32-Gln115 from human PTH expressed in E. coli.
- Clone:
- 24F1
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- PTH (Parathyroid Hormone) is a critical hormone in the regulation of Ca++ homeostasis. The human PTH cDNA encodes 115 amino acids (aa) including a 25 aa signal sequence, a 6 aa propeptide, and an 84 aa mature hormone. Mature human PTH shares 70%, 73%, 88%, 87%, 86%, 86% and 85% aa identity with mouse, rat, canine, equine, bovine, porcine and feline PTH, respectively. Multiple N‑terminal peptides and C-terminal peptides derived from PTH occur naturally in the circulation. PTH aa 32-66, called PTH (1-34) since it represents the first 34 aa of the mature hormone, reproduces all the activity of the full length mature hormone and has been used therapeutically for treatment of osteoporosis. C-terminal peptides mainly oppose the activities of PTH (1-34) and are increased in renal failure. PTH expression is mainly restricted to the parathyroid gland, with minor amounts in the thymus. PTH secretion is enhanced by low Ca++ concentrations and inhibited by FGF-23. In normal human plasma, PTH correlates negatively with active Vitamin D and positively with ionized calcium. Human and other mammalian PTH will bind and stimulate human or rat PTH1R, activating adenylate cyclase and increasing cAMP production. PTH promotes secretion of TRANCE/RANKL and periostin through PTH1R binding on osteoblasts and/or bone marrow stromal cells. TRANCE/RANKL induces differentiation of osteoclasts, which in turn promote release of Ca++ from bone. PTH1R on osteocytes, however, allows PTH to promote bone formation and IGF-1 production. In renal epithelium, PTH promotes conversion of Vitamin D to its active form, lowers Ca++ excretion and increases phosphate excretion. PTH also increases hematopoietic stem cell proliferation and mobilization and induces arterial vasodilation by regulating Ca++ influx in PTH1R-expressing arterial smooth muscle. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human parathyroid gland Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice