Thrombomodulin (TM), Recombinant, Human, His-Tag, GST-Tag

Size

£386.00

SKU:: 051672
Application:: WB
Molecular Weight:: 82.1
Purity:: ≥90%.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to 01-1mg/ml. Do not vortex.
Extra Details:: Source: Recombinant protein corresponding to Ala19~Ser515 from human Thrombomodulin, fused to His-Tag & GST-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~82.1kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.