051394
F-Box Protein 3 (FBXO3), Recombinant, Mouse, His-Tag, GST-Tag
Size
£359.00
- SKU:
- 051394
- Application:
- WB
- Molecular Weight:
- 66.9
- Purity:
- ≥95%.
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder in PBS, pH 7.4, 0.01% SKL, 5% trehalose. Reconstitute with sterile 10mM PBS, pH 7.4 to 0.1-1mg/ml. Do not vortex.
- Extra Details:
- Recombinant protein corresponding to aa159-480 from mouse F-Box Protein 3, fused to His-Tag and GST-Tag at N-terminal, expressed in E. coli. Swiss/Uniprot: Q9DC63 Amino Acid Sequence: LL GSMALSNHYR SEDLLDVDTA AGGFQQRQGL KYCLPLTFCI HTGLSQYIAVEAAEGRNKNE VFYQCPDQMA RNPAAIDMFI IGATFTDWFT SYVNNVVSGGFPIIRDQIFR YIHDPECVAT TGDITVSVST SFLPELSSVH PPHYFFTYRIRIEMSRDALP EKACQLDSRY WRITNAKGDV EEVQGPGVVG EFPIISPGRIYEYTSCTTFS TTSGYMEGYY TFHFLYFKDK VFNVAIPRFH MACPTFRVSIARLEMGPDEY EEMEEEAEEE EEEENDDSAD MDESDESDAD ENESDEGEGEARRRRVFDVP IRRRRCSRLF Predicted Isoelectric Point: 4.6 Predicted Molecular Mass: 66.9kD Accurate Molecular Mass: 75kD as determined by SDS-PAGE reducing conditions. Phenomenon Explanation: The possible reasons that the actual band size differs from the predicted are as follows: 1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Shipping Conditions:
- Blue Ice