Eukaryotic Complement Component 5a (C5a), Recombinant, Mouse, His-Tag

Size

£395.00

SKU:: 051242
Application:: WB
Molecular Weight:: 10.5
Purity:: ≥95%.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:: Source: Recombinant protein corresponding to Asn679~Arg755 from mouse Eukaryotic Complement Component 5a, fused to Hiis-Tag at N-terminal, expressed in 293F cells. Molecular Weight: ~10.5kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0.. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.