Eukaryotic Nerve Growth Factor (NGF), Recombinant, Rat, His-Tag

Size

£426.00

SKU:: 051239
Application:: WB
Molecular Weight:: 26.6
Purity:: ≥95%.
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose, Reconstitute with ddH2O to a concentration of 0.1-0.5mg/mL. Do not vortex.
Extra Details:: Recombinant protein corresponding to Glu19~Gly241 from rat Eukaryotic Nerve Growth Factor, with a N-terminal His-Tag, expressed in 293F cells. Molecular Weight: ~26.6kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.