Complement Component 3 (C3), Recombinant, Bovine, His-Tag

Size

£372.00

SKU:: 050397
Application:: WB
Molecular Weight:: 27.8
Purity:: ≥95%.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to 01-1mg/ml. Do not vortex
Extra Details:: Recombinant protein corresponding to Gly448~Thr665 from bovine Complement Component 3, with a N-terminal His-Tag, expressed in E. coli. Molecular Weight: ~27.8kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.