ATP Binding Cassette Transporter B4 (ABCB4), Recombinant, Mouse, His-Tag

Size

£367.00

SKU:: 049857
Application:: WB
Molecular Weight:: 30.3
Purity:: ≥95%.
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in 20mM Tris, 150mM sodium chloride, pH 8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. Reconstitute with 20mM Tris, 150mM sodium chloride, (pH 8.0) to a concentration of 0.1-1.0mg/ml Do not vortex
Extra Details:: Recombinant protein corresponding to Asn1034~Leu1276 from mouse ATP Binding Cassette Transporter B4, with a N-terminal His-Tag, expressed in E. coli. Molecular Weight: ~30.3kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.