Protein Phosphatase 1, Catalytic Subunit Alpha Isoform (PPP1Ca), Recombinant, Human, His-Tag

Size

£369.00

SKU:: 049607
Application:: WB
Molecular Weight:: 41.1
Purity:: ≥97%.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in 20mM Tris, 150mM sodium chloride, pH 8.0, containing 1mM EDTA, 1mM DTT, 0.01% skl, 5% Trehalose and Proclin300. Reconstitute in 20mM Tris, 150mM sodium chloride (pH 8.0) to a concentration of 0.1-1.0mg/ml. Do not vortex.
Extra Details:: Recombinant protein corresponding to Ser2~Lys330 from human Protein Phosphatase 1, Catalytic Subunit Alpha Isoform, with a N-terminal His-Tag, expressed in E. coli. Molecular Weight: ~41.1kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute in 20mM Tris, 150mM sodium chloride (pH 8.0) to a concentration of 0.1-1.0mg/ml. Do not vortex. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.