Eukaryotic Osteopontin (OPN), Recombinant, Human, His-Tag

Size

£336.00

SKU:: 049109
Application:: WB
Molecular Weight:: 32.3
Purity:: ≥95%. Endotoxin: ≤1EU/ug (LAL)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:: Recombinant protein corresponding to Ile17~Asn287 from human Eukaryotic Osteopontin, with a N-terminal His-Tag, expressed in 293F cells. Molecular Weight: ~32.3kD Applications: Suitable for use as a positive control, immunogen, SDS-PAGE and Western Blot. Other applications not tested Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.