CD14 (Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein)

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£889.00

SKU:: 048959
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant. Endotoxin: ≤0.1EU/ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Thr20-Cys352 from human CD14, expressed in CHO cells.
Clone:: 9L808
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: CD14 occurs both as a secreted protein and as a GPI-linked membrane protein. It plays a critical role in lipopolysaccharide (LPS)-dependent signal transduction. Applications: Suitable for use in Flow Cytometry, Western Blot, Neutralization and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.25ug/ml labels 10e6 cells in human peripheral blood mononuclear cells (PBMCs) Neutralization: Measured by its ability to neutralize lipopolysacharide (LPS)-induced TNF‑ alpha secretion in the THP-1 human acute monocytic leukemia cell line. At 10ug/ml 048959 will neutralize >60% of 0.5ng/ml LPS-induced TNF-alpha secretion. Immunohistochemistry: 5-25ug/ml using immersion fixed paraffin-embedded sections of human tonsil Western Blot: 2ug/ml using human peripheral blood mononuclear cells (PBMC) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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