048948
HN-Tag
Size
£448.00
- SKU:
- 048948
- Purity:
- Purified by immunoaffinity chromatography and ammonium sulphate precipitation.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Conjugated peptide with sequence -CHNHNHNHNHNHNHN
- Format:
- Supplied as a lyophilized powder. Reconstitute with sterile PBS.
- Extra Details:
- Plasmid vectors are commonly used for the expression of hybrid fusion proteins, encoding a protein of interest fused to an epitope tag. Antibodies specific for such fusion protein tags are useful for monitoring expression of the protein of interest, both in cells and in Western blot analysis. Such epitope tags in common use include Myc, GST, His, HA and GFP. HN is a novel series of epitope tags. The tags are encoded by the gateway expression vectors, a group of unique and effective vectors suitable for the production of recombinant tagged fusion proteins. Each expression vector encodes a unique, 12 amino acid HN-Tag epitope sequence. Anti-HN-Tag antibodies are suitable for purification, detection and immunoprecipitation of recombinant fusion proteins containing the respective HN-Tag. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:1000-1:5000 Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice