TrkC (Tyrosine Kinase Receptor C)

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£863.00

SKU:: 048945
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Cys32-Asp428 from human TrkC, expressed in NS0 cells
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. Binding to and activating two unrelated receptor types mediate the biological activities of the neurotrophins. These receptors include the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases. P75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins and transduces cellular signaling pathways that may synergize or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, have been identified. TrkA preferentially binds NGF and NT-3, TrkB preferentially binds BDNF, NT-3, and NT-4/5, and TrkC preferentially binds NT-3. All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain have been described. At the protein sequence level, Trks are highly conserved between species with the extracellular domains of human and mouse TrkC exhibiting 94% amino acid sequence identity. The proteins also exhibit cross- species activity. Although TrkC is widely expressed, the highest levels are found in the nervous system. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.25ug/10e6 cells using human TrkC transfected Baf/3 cells; A549 human lung carinoma cell line Western Blot: 1ug/ml in lysates of human brain (cerebellum) tissue, human brain (hippocampus) tissue, human brain (hypothalamus) tissue, and human brain stem tissue Immunohistochemistry: 5-15ug/ml in immersion fixed paraffin-embedded sections of human brain (hippocampus) and perfusion fixed frozen sections of mouse brain (cortex) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice