Sulf-2 (Extracellular Sulfatase Sulf-2, SULF2, KIAA1247)

Size

£812.00

SKU:: 048942
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human Sulf-2.
Clone:: 2B4
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Sulf-2 is also important in neurological situations including myelination and glial cell differentiation and GDNF activity. Sulf-2 activity is also found in the kidney and important in glomerular basement membrane development and glomerular function. Finally, Sulf-2 is involved in heparan sulfate metabolism. Sulf-2 removes sulfate moieties on heparan sulfate proteoglycans which in turn modulate the heparin binding growth factor signaling activity of the cell. Sulf-2 also impacts extracellular matrix interactions and it is important during development and growth, as well as Wnt signaling and VEGF production by endothelial cells during vascularization. In disease, Sulf-2 expression in cancer is upregulated and inhibition of Sulf-2 limits cancer progression and showed increased apoptosis within tumors, thus Sulf-2 activation is being examined as a potential anti-cancer therapy. In an ELISA anaylsis, this antibody detected Sulf-2, but not Sulf-1 Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml detected Sulf-2 in 10ug of A549 cell lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice