048934
Interferon beta (IFN beta)
Size
£863.00
- SKU:
- 048934
- Purity:
- Purified by immunoaffinity chromatography. Endotoxin: ≤0.1EU/ug (LAL).
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to human IFN-beta, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- IFN-beta (interferon beta; also fibroblast IFN) is a secreted, monomeric 23-24kD member of the alpha/beta interferon family of molecules. It can be produced by almost all cell types in response to bacterial DNA or viral double-stranded RNA and is abundantly secreted by circulating plasmacytoid DCs. IFN-beta drives monocytic transformation into DCs, and it appears to stimulate a B cell switch from from IgM to IgG secretion. Mature human IFN-beta is 166 amino acids (aa) in length (aa22-187) and contains one phosphorylation site at Ser140. There is one potential alternative start site at Met22. Full-length human IFN-beta (aa22-187) shares 47% aa identity with mouse IFN-beta. Applications: Suitable for use in Western Blot and Neutraliztion. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml using recombinant human IFN-beta. Neutralization: Measured by its ability to neutralize IFN‑ beta inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line (1). The ND50 is typically 0.100-1ug/ml in the presence of 50pg/mL Recombinant Human IFN‑ beta. Optimal dilutions to be determined by researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice