HLA-G (HLAG, HLA Class I Histocompatibility Antigen alpha Chain G)

Size

£819.00

SKU:: 048733
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal region of human MHC class I antigen G. The immunogen sequence is conserved in all isoforms.
Clone:: 4H84
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: HLA class I histocompatibility antigen, alpha chain G (UniProt: P17693; also known as HLA G antigen, MHC class I antigen G) is encoded by the HLA-G (also known as HLA-6.0, HLAG) gene (Gene ID: 3135) in human. HLA-G is a single-pass type I membrane glycoprotein that serves as marker of extravillous cytotrophoblasts in placenta and plays a central role in molecule maternal tolerance of the semiallogeneic fetus. It is involved in the presentation of foreign antigens to the immune system. HLA-G is a heterodimer of an alpha chain and a beta chain. Severn different isoforms of HLA-G have been described that are produced by alternative splicing and their distribution is temporally affected during pregnancy in placenta and fetal tissues. HLA-G expression has been reported in renal cell carcinoma where it is exclusively restricted to tumor cells. Its expression is thought to be involved in the impairment of the anti-tumor immunity or resistance to immunotherapeutic agents. HLA-G is synthesized with a signal peptide (aa1-24), which is subsequently cleaved off to generate the mature form, which contains an extracellular domain (aa25-308), a transmembrane domain (aa309-332), and a short cytoplasmic tail (aa333-338). Applications: Suitable for use in Flow Cytometry, Western Blot, ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 1:50 detected HLA-G in human spleen tissue sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice