PSD-95 (Palmitoylated)

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£581.00

SKU:: 048732
Purity:: Purified by Protein A affinity chromatography from animal free supernatant. ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Human
Immunogen:: Palmitoylated PSD-95.
Clone:: PF11 (recomb)
Format:: Supplied as a liquid in PBS. No preservative added.
Extra Details:: PSD-95 is a member of proteins located at a specialized postsynaptic membrane region, called the postsynaptic density (PSD). PSD-95 is the most abundant scaffold protein specifically enriched in the PSD. It contains three PDZ domains, an SH3 domain and a guanylate kinase (GuK)-like domain. Through its PDZ domains, PSD-95 assembles various synaptic components at the PSD including intracellular signaling molecules (e.g. SynGAP and kalirin-7), ion channels (e.g. stargazin/AMPA receptors [AMPARs] and NMDA receptors) and cell adhesion molecules (e.g. neuroligin). PSD-95 plays a primary role in synaptic development and maturation and is regulated by palmitoylation at its N-terminal cysteine residues leading to its postsynaptic targeting. Palmitoylation of PSD-95 is triggered by DHHC2, a plasma membrane-inserted palmitoylating enzyme. Palmitoylated PSD-95 is almost exclusively localized at excitatory synapses in neurons. 048732 is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain. Produced without the use of animals. Purified from HEK 293 cell culture supernatant. Applications: Suitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunocytochemistry (fixed with methanol): 1:1000 Immunohistochemistry (fixed with acetone; does not work with paraformaldehyde): 1:500 Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice