048725
Parvalbumin
Size
£570.00
- SKU:
- 048725
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Chicken/Avian
- Immunogen:
- Full length recombinant protein corresponding to human PVALB, expressed in E. coli.
- Format:
- Supplied as a liquid, 0.02% sodium azide.
- Extra Details:
- Parvalbumin is a low molecular weight cytoplasmic Calcium binding protein containing the “EF hand” Calcium binding motif and is the first protein characterized in this subclass. Parvalbumin is expressed in fast-contracting muscles, in the brain and in some endocrine tissues. In brain it is particularly concentrated in Purkinje cells and interneurons in the molecular layer, but is also found in many cortical GABAergic interneurons. These GABAergic interneurons in most cases express only one of three Calcium binding proteins, namely parvalbumin, calretinin or calbindin. Each type of interneuron has distinct electrophysiological properties and as a result, different types of interneuron can be identified and classified based on their content of these three proteins. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000, recognizes a band at 12kD in skeletal muscle lysates which represents parvalbumin and reacts only with parvalbumin and not the other calcium-binding proteins. This antibody does not recognize parvabumin in rat or mouse brain lysates on western blots. Immunohistochemistry: 1:2000 Immunofluorescence (IC): 1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice