Precision Plus®-CL & SY qPCR Master Mix

Size

£586.00

SKU:: 048713
Storage Conditions:: -20°C
Supplier:: United States Biological
Extra Details:: PrecisionPLUS qPCR Master Mix is our next generation qPCR Master Mix and has an antibody mediated Hot Start mechanism which releases more active enzyme and requires a much shorter activation time. This saves time and the amplification plots have a higher level of fluorescence. This new kit contains a higher fidelity Taq enzyme which further reduces the possibility of primer dimer formation. In addition, we have switched from a chemical hotstart Taq to an antibody modification in PrecisionPLUS OneStep and thus have been able to reduce the enzyme activation time resulting in shorter run times. As a consequence, you will see the same or better data, in a shorter time. The PrecisionPLUS OneStep RT-qPCR Master Mix is a convenient, one step, closed tube reaction which means reduced setup/handling time as well as reduced risk of contamination. Our optimised rapid protocol includes a 10 minute RT step. It is also ideal for RNA pathogen detection. Just add primers and template for a perfect real-time PCR reaction. Suitable Sample Material: All kinds of sample material suited for PCR amplification can be used. Please ensure the samples are suitable in terms of purity, concentration and DNA integrity. Always run at least one negative control with the samples. To prepare a negative control, replace the test sample with RNase/DNase free water. Recommended Accompanying Products: • Primerdesign custom designed real-time PCR primer or primer/probe mixes • Primerdesign Precision nanoscript2 Reverse Transcription kit for production of cDNA template • genesig pathogen detection kits • Primerdesign real-time PCR Internal Control • Primerdesign BrightWhite real-time PCR plasticware Reagents and Equipment To Be Supplied by User: • Real-Time PCR Instrument • Pipettors and Tips • Vortex and centrifuge Storage and Stability: Stored at -20ºC on arrival. Repeated freeze/thawing should be kept to a minimum to maximize performance of this product. Stable for 6 months after reciept.

Shipping Conditions:: Dry Ice