Nup98

Size

£704.00

SKU:: 048706
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: GST-tagged recombinant protein corresponding to human Nup98 near the N-terminus.
Clone:: 2H10
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: The human gene NUP98 (Gene ID 4928; Also known as ADAR2, ADIR2, NUP96, NUP196) encodes the nuclear pore complex protein Nup98-Nup96 (UniProt P52948; also known as GLFG-repeat containing nucleoporin, Nup98-Nup96), which is posttranslationally autocleaved to form Nup98 and Nup96. Cleavage of the Nup98-96 linkage enhances the interaction between Nup98 and Nup96 and promotes localization of the two proteins to the nuclear pore complex (NPC). An alternative splice event generates a mRNA variant (isoform 3; also known as Nup98-specific 1), which expresses only the Nup98 portion of the Nup98-96 mRNA and allows the independent production of Nup98. Unlike Nup96, Nup98 is known to exist in both NPC-bound and non-NPC-associated pool, where it may participate in gene transcription regulations. Nup98 is reported to interact with histone-modifying enzymes CBP/p300 and histone deacetylases (HDACs) through its glycine-leucine-phenylalanine-glycine (GLFG) repeats. NUP98 gene is also involved in chromosomal translocations, resulting in Nup98 translocation fusions identified as potent transcription factors that induce acute myeloid leukemia (AML) with a particularly poor patient prognosis. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml detected Nup98 in 10ug of HeLa nuclear extract. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice