Heat Shock Protein 90, beta (HSP90)

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£639.00

SKU:: 048673
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human HSP90 beta.
Clone:: H9010
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Extra Details:: The 90kD heat shock protein (HSP90) is a molecular chaperone that keeps a target protein in a folding-competent state. Hsp90 is ubiquitously expressed and abundant, and it participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase. In its ATP-bound state, Hsp90 interacts with the co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. Hsp90 is highly conserved between species; mammalian proteins demonstrate 60% amino acid identity with the corresponding yeast and 78% identity with the corresponding Drosophila proteins. Two isoforms of HSP90, alpha and beta, are expressed in the cytosolic compartment.6 The amino acid sequence of human HSP90 alpha is 85% homologous to HSP90 beta, but HSP90 alpha exists predominantly as a homodimer while HSP90 beta exists mainly as a monomer. Applications: Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot, Immunocytochemistry and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: 1:100 Western Blot: 1:2500, 1ug/ml was sufficient for detection of HSP90beta in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG (HRP) as the secondary antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice