048660
Insulin-Like Growth Factor I Receptor, alpha (IGF1Ra)
Size
£793.00
- SKU:
- 048660
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- IGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA. Epitope: aa283-440 (exon 4-6).
- Clone:
- 24-31
- Format:
- Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
- Extra Details:
- Both IGF-1R and insulin receptor are synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The a-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity. It is assumed that, as for the insulin receptor, the tyrosine kinase activity is essential for IGF-1 action. Applications: Suitable for use in Immunoprecipitation, Immunohistochemistry, Inhibition and as a weak IGF-like Agonist in Bioassays. Other applications not tested. Recommended Dilutions: Immunoprecipitation: 2ug/mg protein lysate. Use Protein G. Immunohistochemistry (Frozen and formalin/paraffin): 2-4ug/ml for 30 min. at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min. followed by cooling at RT for 20 minutes. Please note that EDTA is better than citrate for epitope unmasking. Bioassays: Thymidine incorporation into DNA Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice