Dinitrophenol (DNP)

Size

£704.00

SKU:: 047612
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: DNP-KLH
Clone:: SPE-7
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: Dinitrophenyl (DNP) is a hapten that is often used for labeling primary or secondary probes in immunological assays. Hence, anti-DNP antibody is a useful tool for the detection and analysis of target molecules. IgE gene is encoded on human chromosome 14. IgE is the least circulating antibody, but critical for eliciting hypersensitivity reaction against allergens. Applications: Suitable for use in ELISA. Other applications have not been tested. Recommended Dilutions: Indirect ELISA: 0.05-0.1ug/mL using using 0.5ug/well DNP-BSA as the coating antigen and 1ug/mL Monoclonal Anti-Mouse IgE, Peroxidase conjugate as the detector antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.