Coronavirus MHV-JHM Nucleocapsid, Mouse

Size

£754.00

SKU:: 047566
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Robb/Lampert strain of MHV-JHM (live whole virus).
Clone:: 1-16-1
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Extra Details:: Nucleoprotein (UniProt: C0KYZ4; also known as Nucleocapsid protein, NC, Protein N) is encoded by the N gene(Gene ID: 1489757) in Murine coronavirus (MHV-JHM.IA). The murine coronavirus mouse hepatitis virus (MHV), a member of the Coronaviridae family, is an enveloped virus with a single-stranded, positive-sense RNA genome. The MHV-JHM strain is shown to be highly neurovirulent and can spread in the brain in mCEACAM1a independent manner. MHV-JHM infection in susceptible weanling mice is reported to result in an encephalomyelitis when neurons, astrocytes, and oligodendrocytes are infected. MHV infection of the murine central nervous system has also been used as a model for the study of chronic demyelinating diseases. The nucleocapsid protein (N) is a basic RNA binding protein that is highly conserved among MHV strains and plays a major role in the extent of neurovirulence. It also plays structural roles by both complexing with genome RNA to form the viral capsid and interacting with the viral membrane protein (M) during virion assembly. It has three conserved regions (I, II, and III) that are separated by two hypervariable regions. It is reported to associate with microtubules, suggesting a possible role for N in trafficking and axonal transport in neurons (Ref.: Cowley, TJ., et al. (2010). J. Virol. 84(4); 1752 1763; Miura, TA., et al. (2008). J. Virol. 82(2); 755-763). Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 1:500 detected Murine Coronavirus MHV-JHM nucleocapsid in Mouse delayed brain tumor (DBT) cell lysates. Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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