HLA Class 1 Antigen G (HLA Cass I Histocompatibility Antigen alpha Chain G, HLA G Antigen, HLA G, HLAG, HLA-G, HLA-6.0, Major Histocompatibility Complex Class I G, MHC-G) (APC)

Size

£735.00

SKU:: 046287
Application:: Flow Cytometry
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human HLA-G refolded with beta2-microglobulin and peptide.
Clone:: MEM-G/9
Format:: Supplied as a liquid in PBS, pH7.4, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Extra Details:: HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45kD and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1-5ug/ml using JEG-3 human choriocarcinoma cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice