046270
AHNAK1 (Desmoyokin)
Size
£512.00
- SKU:
- 046270
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bacterially expressed fragment of N-terminal domain of human AHNAK1.
- Clone:
- EM-09
- Format:
- Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide
- Extra Details:
- AHNAK1 (Desmoyokin) is a large (700kD) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex. Applications: Suitable for use in Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunocytochemistry permeabilization is required. Immunohistochemistry: frozen Optimal dilutions to be determined by the researcher. Positive Control: HeLa human cervix carcinoma cell line Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice