046261
Sarcolipin (SLN)
Size
£812.00
- SKU:
- 046261
- Application:
- WB
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- KLH-conjugated linear peptide corresponding to the C-terminus of human Sarcolipin. Species Sequence Homology (100%): bovine, human, mouse and monkey
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
- Extra Details:
- Sarcolipin (UniProt: O00631; also known as SLN) is encoded by the SLN gene (Gene ID: 6588) in human. Sarcolipin is a single-pass membrane protein that contains a cytoplasmic domain (aa 1-7), a transmembrane domain (aa 8-26), and a lumenal domain (aa 27-31). SLN is a regulator of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) activity in muscle and plays a role in regulating muscle thermogenesis and metabolism. It increases the heat generated by SERCA by partially uncoupling Ca2+ re-sequestration from ATP hydrolysis in muscle and contributes to non-shivering thermogenesis. This heat generating process is mechanistically distinct from and unrelated to Ca2+-linked thermogenic processes in skeletal muscle. It acts by reducing the apparent affinity of the ATPase for Ca2+. SLN is also reported to modulate calcium re-uptake during muscle relaxation and play an important role in calcium homeostasis in muscle. Higher levels of SLN have been reported in patients with Duchenne muscular dystrophy and reducing SLN levels improves dystrophic pathology. (Ref.: Campbell, KL., and Dicke, AA (2018). Front. Physiol. 9; Article 714; Voit, A., et al. (2017). Nat. Commun. 8; Article 1068). Applications: Suitable for use in Western Blot and Peptide Inhibition Assay. Other applications not tested. Recommended Dilutions: Western Blot: 1:500 detected Sarcolipin in rabbit quadriceps muscle tissue lysate. Peptide Inhibition Assay: Sarcolipin detection in rabbit quadricept tissue lysate was completely inhibited by preblocking with 1mg of antigen peptide for 1 hour at 37°C Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice