IgG1, Mouse, Negative Isotype Control

Size

£448.00

SKU:: 046257
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Clone:: PPV-06
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10ug/ml of incubation mixture), change the conditions of your experiment to reduce the background. Applications: Suitable for use as a Immunohistochemistry, Flow Cytometry, Immunocytochemistry, ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommeded Dilutions: Immunohistochemistry: Frozen and formalin/paraffin sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice