046247X

Rabies Virus, Glycoprotein (Serum Free)

Size

£787.00

SKU:: 046247X
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Glycoprotein of Evelyn Rokitniki Abelseth(ERA) SAD derived RABV strain.
Clone:: 1112-1
Format:: Supplied as a liquid.
Extra Details:: Glycoprotein (UniProt: P03524) is encoded by the G gene in Rabies virus, strain ERA. Glycoprotein is a single-pass type I membrane protein that belongs to the lyssavirus glycoprotein family. It attached the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. The muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) are reported to bind glycoprotein and thereby facilitate rabies virus entry into cells. Glycoprotein is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off in the mature form. Its structure includes a virion surface (aa 20-459), helical region (aa 460-480) and an intravirion (aa 481-524). Arginine 352 is reported to be involved in Rabies virus pathogenicity and its mutation attenuates the virus. Glycoprotein is glycosylated and palmitoylated by host and glycosylation is considered to be crucial for glycoprotein export at the cell surface. 046247X is a serum-free version and is shown to neutralize Rabies Virus, CVS-11 strain. Applications: Suitable for use in Flow Cytometry, ELISA and Neutralization. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2ug detected serum free Rabies virus in L929 cells expressing Rabies virus glycoprotein. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice