Rabies Virus, Strain CVS-11

Size

£761.00

SKU:: 046246
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Beta-propiolactone inactivated Rabies virus, strain CVS-11 (RABV).
Clone:: 509-6
Format:: Supplied as a liquid in PBS. No preservative added.
Extra Details:: Glycoprotein (UniProt: O92284) is encoded by the G gene in Rabies virus, strain CVS-11. Glycoprotein is a single-pass type I membrane protein that belongs to the lyssavirus glycoprotein family. It attached the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. The muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) are reported to bind glycoprotein and thereby facilitate rabies virus entry into cells. Glycoprotein structure includes a virion surface (aa 20-459), helical region (aa 460-480) and an intravirion (aa 481-524). Arginine 352 is reported to be involved in Rabies virus pathogenicity and its mutation attenuates the virus. Glycoprotein is glycosylated and palmitoylated by host and glycosylation is considered to be crucial for glycoprotein export at the cell surface. 046246 is shown to neutralize all fixed and street rabies virus strains except some virus isolates from bats. The conformational antigenic site I for this clone is located at aa231. (Ref.: Wiktor, TJ and Koprowski, H (1980). J. Exp. Med. 152: 99-112; Marissen, WE et al (2005). J. Virol. 79(8): 4672 4678). Applications: Suitable for use in Flow Cytometry, ELISA and Neutralization. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1ug detected glycoprotein of Rabies virus in 10e6 L929 cells expressing the rabies protein. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice