Glyceraldehyde 3 Phosphate Dehydrogenase (GAPDH, Glyceraldehyde-3-phosphate Dehydrogenase, G3PD, GAPD)

Size

£512.00

SKU:: 046225
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human CD4-positive lymphocytes
Clone:: FF26A
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is an intracellular homotetramerizing enzyme, which catalyzes oxidation of glyceraldehyde-3-phosphate into 1,3-bisphosphoglycerate during glycolysis, and has also functions in DNA replication, gene expression, and microtubule bundling. As it binds to many proteins, its functions are very broad. GAPDH plays also roles in cancer progression, apoptosis, Huntington disease and Alzheimer´s disease. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Western Blot: 1ug/ml; both reducing and non-reducing conditions; Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.