046219-PE
Ki-67 (Antigen Identified by Monoclonal Antibody Ki-67, KIA, MKI67, Proliferation Related Ki-67 Antigen) (PE)
Size
£581.00
- SKU:
- 046219-PE
- Application:
- Flow Cytometry
- Purity:
- Purified
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Nuclei of the Hodgkin lymphoma cell line L428
- Clone:
- Ki-67
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345kD and 395kD), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells of 100ul whole blood or 1x10e6 cells in suspension Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice