Neurofilament Light Chain (NF-L, NEFL)

Size

£544.00

SKU:: 046201
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: C-terminal peptide corresponding to aa514-542 of human NF-L protein, GEEEDTKESEEEEKKEESAGEEQVAKKKD with an N-terminal C for coupling to KLH.
Clone:: 6H112
Format:: Supplied as a liquid in PBS, 0.03% sodium azide, 50% glycerol.
Extra Details:: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other filament proteins may be included also. The major function of neurofilaments is likely to control the diameter of large axons (1). NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at 68-70kD with some variability across species. Antibodies to NF-L like 046201 are useful for identifying neuronal cells and their processes in cell culture and sectioned material. NF-L antibody can also be useful for the visualization of neurofilament rich accumulations seen in many neurological diseases, such as Lou Gehrig’s disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease and others (2-4). Much interest has recently been focused on the detection of NF-L released from neurons into blood and CSF as a surrogate marker of primarily axonal loss in a variety of types of CNS injury and degeneration (5). Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence/ Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:5000 Immunohistochemistry (PFA fixed): 1:2000-1:4000 Immunofluorescence (IC): 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice