TNF alpha (Tumor Necrosis Factor alpha, TNFA, DIF, TNLG1F)

Size

£512.00

SKU:: 046194
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human Tumor Necrosis Factor a.
Clone:: MAb11
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis. Applications: Suitable for use in ELISA, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilution: Immunohistochemistry: paraformaldehyde-fixed, saponin-treated frozen tissue sections. Flow Cytometry: Intracellular staining. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.