046184-PE

TCR V alpha 24 J alpha 18 (PE)

Size

£530.00

SKU:: 046184-PE
Application:: Flow Cytometry
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Clone:: 6B11
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with R-Phycoerythrin (PE).
Extra Details:: TCR alpha V24-J18 chain is a marker of invariant NK T cells (iNKT). This chain pairs mostly with TCR beta V11 chain, and together they interact with glycolipide-loaded CD1d. This interaction results in iNKT activation and cytokine production (IFN gamma, IL-4, IL-13), which takes important part in immune regulation and host response to pathogens and cancer(support of both Th1 and Th2 way), although iNKT cells represent less than 1% of total peripheral T cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.