CD95 (FAS1, APT1, APO-1, FASTM, ALPS1A, TNFRSF6)

Size

£512.00

SKU:: 046179
Application:: Flow Cytometry
Purity:: Purified by ammonium sulfate precipitation.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: P815 cells transfected with human CD95
Clone:: EOS9.1
Format:: Supplied as a liquid in TBS, pH 8.0, 15mM sodium azide.
Extra Details:: CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 2-6ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.