Catalase

Size

£544.00

SKU:: 046174
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Full length recombinant protein corresponding to human Catalase, expressed in E. coli.
Clone:: 6H14
Format:: Supplied as a liquid in PBS, 5mM sodium azide, 50% glycerol.
Extra Details:: Catalase is an enzyme found in all living organisms, including animals, plants, and microorganisms. In eukaryotes it is localized in peroxisomes, which are small, membrane-bound organelles responsible for various metabolic reactions in cells. Catalase catalyses the breakdown of hydrogen peroxide (H2O2) into water (H2O) and oxygen (O2). Hydrogen peroxide is a byproduct of various biochemical reactions in cells and can be harmful if allowed to accumulate, as it can modify and damage cellular proteins, lipids and nucleic acids (1,2). Catalase therefore prevents oxidative stress and maintains cellular health. Catalase forms a tetramer in vivo, and, like hemoglobin and the cytochromes, each subunit contains an iron containing heme group. The enzyme catalase is widely studied in biochemistry as liver is an unusually rich source of the native protein which allowed purification, amino acid sequence determination and crystallization in the 60s and 70s. Catalase activity is important for the regulation of cellular physiology and changes in aging and diseases associated with oxidative stress. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500 Immunocytochemistry/Immunofluorescence: 1:200 Immunohistochemistry (neutral buffered formalin fixed and paraffin embedded specimens): 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice