Choline Acetyltransferase (CAT, CHAT) (Not for Export EU)

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£614.00

SKU:: 046166
Purity:: Serum.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human choline acetyltransferase.
Format:: Supplied as a liquid. No preservative added.
Extra Details:: Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer's disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999). Applications: Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (paraffin): 1:100 Western Blot: 1:1000 Immunoprecipitation: 1:200 immunoprecipitated 68kD active ChAT from brain and placenta. Also precipitates a 27kD protein that is co-expressed with ChAT (but lacking ChAT activity) in some cholinergic neurons of the central nervous system of humans and possibly other mammals. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice