046162
Integrin alphaV, beta 5
Size
£863.00
- SKU:
- 046162
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant. Endotoxin: ≤0.10EU/ug (LAL)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- HT1080 human fibrosarcoma cell line
- Clone:
- P5H9
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Integrins are heterodimeric receptors comprised of an alpha and a beta subunit. Integrin alpha V (CD51) associates with several different beta subunits, but Integrin beta 5 associates exclusively with the alpha V subunit. Integrin alpha V beta 5, also known as Integrin alpha V beta S and Integrin alpha V beta 3B, is a transmembrane heterodimeric protein that functions as a receptor for Vitronectin. It is expressed on hepatoma cells, fibroblasts and carcinoma cells. Applications: Suitable for use Flow Cytometry, Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 8-25ug/ml in HT1080 human cell line Flow Cytometry: 2.5ug/10e6 cells in MCF‑7 human breast cancer cell line Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice