IRE1, phosphorylated (Ser724) (ERN1, IRE1a, Endoplasmic Reticulum-to-Nucleus Signaling 1, Inositol-Requiring Protein 1, Serine/Threonine Protein Kinase/Endoribonuclease IRE1)

Size

£696.00

SKU:: 046160
Application:: WB
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser724 of mouse IRE1, conjugated to KLH. Species sequence homology: human
Format:: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Extra Details:: IRE1, inositol requiring 1 protein, is an ER transmembrane sensor that activates unfolded protein response (UPR) to maintain the ER and cellular function (Chen et al, 2013). The activation of UPR involves three signaling pathways, IRE1, PERK, and ATF6, which are crucial to returning protein homoeostasis to levels of non-stressed cells (Stewart et al, 2012). Changes in ER homeostasis causing unfolded protein buildup can be due to Ca2+ depletion, hypoxia, altered glycosylation, and viral infection triggering the UPR and activation of IRE1 (Stewart et al, 2012). UPR dysfunction plays an important role in the pathogenesis of neurodegenerative diseases including Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis and Huntington's disease, which is characterized by the accumulation and aggregation of misfolded proteins (Xiang C et al, 2017). The phosphorylation of IRE1 at ser724 may play a significant role in understanding these diseases. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:10000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice