046152
Adipophilin (Perilipin-2, Adipose Differentiation-Related Protein, ADRP, PLIN2, ADFP) (Not for Export EU)
Size
£935.00
- SKU:
- 046152
- Purity:
- Serum.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Guinea Pig
- Immunogen:
- Synthetic peptide corresponding to the C-terminal (a423-437) of human Adipophilin.
- Format:
- Supplied as a liquid in 0.09% sodium azide.
- Extra Details:
- Adipophilin/ ADRP/ PLIN2 is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (see Heid et al. 1998 & 2013; for review see Targett-Adams et al. 2003); inducible by etomoxir. Enhanced expression of Adipophilin/ ADRP/ PLIN2 is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study the virus entry via lipid droplets (see Hope et al. 2002). Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative. Also positively stained are lipid-storing CD 68-positive macrophages. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry/Immunofluorescence. Other applicatons not tested. Recommended Dilutions: Western Blot: 1:500-1:2000 Immunohistochemistry (Frozen, Paraffin embedded tissue after microwave treatment): 1:100; Incubate for 1hr at RT or overnight at 4°C. Immunocytochemistry/Immunofluorescence: 1:100 Optimal dilutions to be determined by the reseracher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice