Adipophilin (ADFP, Adipose Differentiation-related Protein)

Size

£944.00

SKU:: 046150
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to aa5-27 from the N-terminus of human Adipophilin. MW: 48.1kD. Apparent MW: 52kD by SDS-PAGE.
Clone:: AP125
Format:: Supplied as a liquid in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide.
Extra Details:: Adipophilin is a ubiquitous compound of lipid droplets. Adipophilin is present in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines and is inducible by etomoxir. Enhanced expression of ADRP is a useful marker for pathologies characterized by increased lipid droplet accumulation; such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It can also be a potent marker for atherosclerosis. It can also be used to study the virus entry of e.g. HCV via lipid droplets. Adipophilin is localized to glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver. Adipocytes do not display staining. Lipid-storing CD68-positive macrophages do positively stain for adipophilin. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen): 1:10-1:100 (0.5-5ug/ml) Immunohistochemistry (paraffin): 1:10-1:100 (0.5-5ug/ml microwave treatment recommended) Western Blot: 1:50-1:100 (0.5-1ug/ml) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice