Tet Repressor (TetR, Tetracycline Repressor Protein Class D)

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£614.00

SKU:: 046146
Application:: WB
Purity:: Purified by Immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to amino acids from the N-terminal region of E. coli Tet repressor
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: The tet repressor protein (TetR) regulates transcription of a family of tetracycline resistance determinates in Gram-negative bacteria. The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications. In mammals and in a large variety of organisms, however eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.